Comparative Study of the Inhibitory Activities of Ocimum gratissimum and Nepeta cataria against Salmonella Enterica Serovar Typhi and their Larvicidal Effect against Anopheles Gambiae

  • Iheukwumere I.H. Department of Microbiology Chukwuemeka Odumegwu Ojukwu University Uli, Anambra, Nigeria
  • Ejike C.E. Department of Medical Microbiology Chukwuemeka Odumegwu Ojukwu University Uli, Anambra, Nigeria
Keywords: Phytochemical, Antibacterial, Larvicidal, Typhoid Fever and Pesticide


This study was carried out to evaluate the antibacterial and larvicidal activity of Ocimum gratissimum and Nepeta cataria against Salmonella enterica serovar Typhi (S. Typhi) and Anopheles gambiae. S. Typhi was isolated from raw egg using Salmonella Shigella Agar (SSA), characterized and identified using Gram staining, colonial description and biochemical characteristics. The phytochemical constituents of the leaves extracts were determined quantitatively using spectrophotometric method. The antibacterial activity was carried out using agar-well diffusion method. Tube dilution method was used to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) using double-fold serial dilution at concentration 25mg/ml to 400mg/ml. The larvicidal activity of the leaves extracts was carried out by subjecting A. gambiae larvae to varied concentration of the leaves extracts using in vitro assay at room temperature for 72 h. The phytochemical analysis of the leaves extracts revealed the presence of tannins, saponins, alkaloid, flavonoids, steroids, glycosides, phenolics and essential oil. The antibacterial activities of the leaves extracts showed that ethanol and aqueous extracts of N. cataria (16.30mm, 8.30mm) exhibited slightly higher activity than O. gratissimum (15.70mm,7.67 mm) and their activities differed significantly (p≤0.05) from that of ciprofloxacin (21.30 mm). The result of MICs and MBCs of the leaves extracts of N. cataria and O. gratissimum showed that the N. cataria leaf extract exhibited more inhibitory activities against the tested organism. The larvicidal activities of the leaves extracts at doses (0.1ml and 0.2ml) showed that O. gratissimum (40%, 46.67%) differed significantly by (p≤0.05) from N. cataria (33.3%, 46.67%) at their lower doses. The result of LT50 and LT90 of O. gratissimum (70,116) and N. cataria (71,117) revealed their pronounced larvicidal activities against A. gambiae. This study suggests that O. gratissimum and N. cataria leaves extracts could be used as alternative therapy for typhoid fever, and as pesticides for A. gambiae larvae.


Abubakar, E. M. (2009). Antibacterial activity of crude extracts of euphorbia hirta against some bacteria associated with enteric infection. Journal of Medicinal Plant Resistance 3(7): 498–505.

Adebolu, T. T., Adeoye, O. O. and Oyetaye, V. O. (2011). Effect of galic (Allium sativum) on Salmonella typhi infection, gastrointestinal flora and heamatological parameters of albino rats. African Journal of Biotechnology 10 (35): 6804–6808.

Adewole, A., Oderinde, A., Banlade, O. Faparus, F. and Oyede, R. T. (2012). Larvicidal activities of three plants extracts of common wire weed (Sida acuta), catnip (Nepeta cataria) and neem (Azadrichta indica) against the larva of mosquito (Anopheles gambiae). Academia Journal of medicinal plants 1(2): 37–40.

Bandh, A. S., Lone, A. B., Chrishti, Z. M., Kanti, N. A., Gonsii, A. B. and Saleem, S. (2011). Evaluation of anthelminthic and antimicrobial activity of the methanolic extracts of Nepetacataria4 (8): 29–135.

Cabarera, R., Luiz, J., Macro, F., Oliveira, I., Arrayo, M., Aladnena, A., Usera, M., De Anta, M.T., Gascon, J. and Vila, J. (2004). Mechanism of resistance to several antimicrobial agents in Salmonella clinical isolates causing travellers’diarrhea. Antimicrobial Agents of Chemotherapy 48(10): 3934–3939.

Cavalcanti, E. S. B., Morris, S. M., Lima, M. A. A. and Sanitaria, E. W. P. (2004). Larvicidal activity of essential oils from Brazilian plant against Aedesaegyptic (L). Memorias De Institute Oscoaldo Cruz 99:541–544

Chao-Hsun, J., Yu-chun, H., Yu-fen, C. and Ming-Hsian, C. (2010). Foam properties, detergent abilities and long term preservative efficacy of the saponins from saponin adusmukorossi. Journal of Food and Drug analysis 18:155–160.

Crum, N. F. (2003). Current trends in typhoid fever. Current Gastroenterology 5:279–286.

Dada and Komolafe, O. I. (2013). In vitro evaluation of the inhibitory effect of Ocimum gratissimum against Salmonella typhi. International Journal of Pharmaceutical and Biological Research 4(4): 126–129.

Das, N. G., Goswani, D. and Rabha, B. (2004). Preliminary evaluation of mosquito larvicidal efficacy of plant extracts. Journal of Vector Borne Diseases 44:145–148.

Iheukwumere, I. H. and Umedum, C. U. (2013). Effect of Gossypium hirsutum leaf extracts on Gram negative bacteria isolated from the cervix of females with unexplained infertility. African Journal of Science 14(1): 3261–3270.

Iheukwumere, I., Nwachukwu, C. N., Kanu, M. A. (2013). Manifestation, management and diagnostic challenges of malaria and typhoid. Malaria Chemotherapeutic Control Elimination 2:109–110.

Innocent, E., Cosam, C.J., Nicholas, K. G. and Mainen, J. M. (2008). Mosquito larvicidal constituent from lantana viburnoides. Journal of Vector Borne Disease 45:240–244.

Kotzekidou, R. Giannakidis, P. and Boulamatsis, A. (2008). Antimicrobial activity of some plant extracts and essential oils against food borne pathogens in vitro and on the fate of inoculated pathogen in chocolate. Food Science of Technology 41: 119–127.

Nwobu, R. A. U., Uzochukwu, I. C. and Okoye, E. I. (2010). Phytochemical analysis and antimicrobial activity of Hyptissurveoleus.Medicinal Plant Phytochemicals Pharmacological Therapentices1:390–396.

Odalo, C., Mutua, A., Kindt, R., Jamnadass, R., Simons, A. (2005). Agrofores tree Database. A tree reference and selection guide version 4.0.

Odalo, J., Omalo, M. M., Angira, J., Njeru, P., Ndiege, I. and Hassanali, A. (2005). Repellency of essential oils of some plants from the kanyan coast against Anopheles gambiae. ActaTropica, 95:210–218.

Ofoegbu, U. P., Onyedineke, E. N., Nwokeji, C., Esie, G. N. and Isibor, N. G. (2013). Laboratory evaluation ethanolic and methanolic extracts of Ocimumgratissimum against larva of Anopheles gambiaeand non-target organism. Muris Entomology and Zoology 8(1):185–190.

Okigbo, R. N., Okeke, J. J., and Madu, N. C. (2010). Larvicidal effects of Azadirachtaindica, Ocimumgratissimum and Hyptissuaveotensagainst mosquito larvae. Journal of Agricultural Technology 6 (4): 703–719.

Omojate, G. C., Enwa, F. O., Jewo, A. O. and Eze, C. O. (2014). Mechanism of antimicrobial actions of phytochemicals against enteric pathogen. AReview.Journal of Pharmaceutical, Chemical and biological sciences 2(2):77–85.

Oparaocha, E. T. (2008). Preliminary study on mosquito repellant and mosquitocidal activities of Ocimumgratissimum (Liston) grown in eastern Nigeria. Journal of Vector Borne Diseases 44(4):328–334.

Parekh, J., Jadeja, D., Chanda, S. (2005). Efficacy of aqueous and methanol extracts of some medicinal plants for potential antibacterial activity. Turk. Journal of Biology 29:203–210.

Raddy, P. J., Karishma, D., Munthy, U. S. and Jamil, K. (1992). A microcomputer FORTRAN program for rapid determination of lethal concentration of biocides in mosquito control. Cabios8:209–213.

Sheu, A., Umana, U. E., Timbuak, J.A., Hanman, Q., Lalymus, S. A. and Ikeyemba, D. T. (2012). Effect of aqueous ethanolic extracts of Ocimumgratissimum on the histology of spleen haematological indices of wister rats and its antimicrobial properties. Asian Journal of Medicinal Sciences 4(4):130–133.

Sofowona, A. (2003). Medicinal plants and traditional medicine in Africa.Spectrum book cid, Ibadan. Pp 55–71.

Sulaurmar, K., Perich, M. J. and Boobar, L. R. (1991). Botanical derivative in mosquito control. Journal of the American Mosquito Control Association 7:210–237.

Tsao, R., Roman, C. F. E., Peterson, C. J. and Coast, J. R. (2002). Plant growth regulatory effect and insecticidal activity of extracts of tree of heaven (Atlanthusaltissima). BMC Ecology 2:1–18.

Uneke, C. J. (2008). Concurrent malaria and typhoid fever in the tropics. The diagnostic challenges and public health implication. Journal of Vector Borne Diseases 45:135–142.
How to Cite
I.H., I., & C.E., E. (2018, April 6). Comparative Study of the Inhibitory Activities of Ocimum gratissimum and Nepeta cataria against Salmonella Enterica Serovar Typhi and their Larvicidal Effect against Anopheles Gambiae. African Journal of Education,Science and Technology, 3(4), pp 16-24. Retrieved from