Vesicular Arbuscular Mycorrhizal Inoculation Influences Growth, Nutrient Absorption and Hyphae Colonization of Rough Lemon (Citrus limon) Seedlings

  • D. K. Chebet 1Seeds, Crops and Horticulture Science Department, University of Eldoret
  • F. K. , Wanzala Horticulture Department, Jomo Kenyatta University of Agriculture and Technology, Kenya
  • W. Kariuki Scotts Christian University, Kenya
Keywords: Rough lemons, Vesicular arbuscular fungi, phosphorus, potassium, colonization

Abstract

Many researchers continue to illustrate the benefits of Vesicular Arbuscular Mycorrhiza fungi on crop output, particularly under unfavourable growth environment. In Sub Saharan Africa, mycorrhizae research on economically important fruit seedlings have attracted inadequate attention. The aim of this study was to determine the effect of Vesicular Arbuscular mycorrhiza fungi on the growth, nutrient absorption, and hyphal colonization of rough lemon (Citrus limon) seedlings grown in low phosphorus sand culture and low nutrient sand/soil planting media. The study showed that in non-inoculated plants increased in the corresponding P levels in sandy media as well as in the low sand/soil media in VAM inoculation with respect to lemon seedlings, the number of leaves and the area of the feed and thickness of the stem. The fresh and dry weights of roots, leaves and stems, and the leaf concentration of phosphorus, nitrogen and potassium were also increased by VAM inoculation. Inoculation also enhanced mycorrhizae hyphal colonization of roots and upraised the root absorptive surface area. This study demonstrates that VAM inoculation improves the competence of lemon seedlings to uptake and use scarce soil nutrients thereby enhancing seedling performance. Being a cheap and readily available technology, VAM incorporation is recommended in nursery propagation of seedlings to ensure a good start of clean and healthy fruit seedlings

References

Al-Karaki, G.N. 2013. The effect of arbuscular mycorrhizal fungi on the establishment of sour orange (Citrus aurantium) under different levels of phosphorus. VII.
International symposium on mineral nutrition of fruit crops book series. Acta Horticulturae 984:103-108.

Bagayoko, M., George, E. Römheld, V. and Buerkert, A. 2000. Effects of mycorrhizae and phosphorus on growth and nutrient uptake of millet, cowpea and sorghum on a West African soil. Journal of Agricultural Science 135:399-407

Cardoso, I.M. and Kuyper, TW. 2006. Mycorrhizas and tropical soil fertility: nutrient management in tropical agroecosystems. Agriculture Ecosystem Environment 116:72-84.

Chebet D.K., W. Kariuki., L. Wamocho and F. Rimberia 2020b. Effect of Arbuscular mycorrhizal inoculation on growth, biochemical characteristics and nutrient uptake of passion fruit seedlings under flooding stress International Journal of Agronomy and Agricultural Research (IJAAR), 16(4):24-31

Chebet D.K. F. K. Wanzala and L.S. Wamocho 2020. Effect of Arbuscular Mycorrhizal inoculation on Biomass, Nutrient Uptake, Root Infectivity and Soil Colonization of Papaya (Carica papaya L.) Seedlings”, International Journal of Environment, Agriculture and Biotechnology 5(5):1393-1388

Cruz, A.F., Ishii, T. and Kadoya K. 2000. Effect of arbuscular mycorrhizal fungi on tree growth, leaf water potential and levels of 1-aminocyclopropane-1-carboxylic acid and ethylene in the roots of papaya under water stress conditions. Mycorrhiza 10: 121-123

Dalgaard, T., Hutchings, N.J., and Porter, JR. 2003. Agroecology, scaling and interdisciplinarity. Agriculture Ecosystem Environment 100:39-51.

Elsen, A., Baimey, H., Swennen, R. and De Waele D. 2003. Relative mycorrhizal dependency and mycorrhiza-nematode interaction in banana cultivars differing in nematode susceptibility. Plant and Soil 256: 303-313.

Estrada-Luna, A. A., Davies, Jr. F. T. and Egilla J. N. 2000. Mycorrhizal fungi enhancement of growth and gas exchange of micro-propagated guava plantlets (Psidium guajava L.) during ex vitro acclimatization and plant establishment. Mycorrhiza 10:1-8.

Fidelibus, M.W., Martin, C.A. and Stutz J.C. 2001. Geographic isolates of Glomus increase root growth and whole-plant transpiration of Citrus seedlings grown with high phosphorus. Mycorrhiza 10: 231-236.

Fogg D.N. and N.T. Wilkinson 1958. The colorimetric determination of phosphorus” Analyst 83:406-414

Gerdemann, J.W. and Nicolson, T.H. 1963. Spores of mycorrhizal endogone species extracted from soil by wet-sieving and decanting. Transactions of the British Mycological Society 1963:235-244.

Giovanetti, M. and Mosse, B. 1980. An evaluation of techniques for measuring vesicular-arbuscular infection in roots. New Phytol. 84: 489-500.

Guissou, T. 2009. Contribution of arbuscular mycorrhizal fungi to growth and nutrient uptake by jujube and tamarind seedlings in a phosphate (P)-deficient soil. African Journal of Microbiology Research 3:297-304.

Ishii, T., Shreshta, YH. and Kadoya, KH. 1992. VA mycorrhizal fungi in citrus soils and the relationship between soil factors and number of spores. Journal of Japan Society of Horticultural Science 61:166-167

Khade S.W and B. F. Rodrigues 2009. Studies on Effects of Arbuscular Mycorrhizal (Am.) Fungi on Mineral Nutrition of Carica papaya L. Notingham Botanical and Horticulture Agrobot. Cluj, 37(1):183-186.

Little, T.M. and Hills, F.J. 1978. Agricultural experimentation. Wiley, New York.

Michelson, A. 1992. Mycorrhiza and root nodulation in tree seedlings from five nurseries in Ethiopia and Somalia. For. Ecol. Manage. 48: 335-344.

Maqueda C. and E. Morillo 1990. Determination of calcium by atomic-absorption spectrometry in samples dissolved by acid mixtures. Fresenius J Anal Chem 338:253–254 https://doi.org/10.1007/BF00323018

Millner, P.D. and Kitt, D.G. 1992. The Beltsville method for soilless production of vesicular-arbuscular mycorrhizal fungi. Mycorrhiza 2:9-15.

Muok, O.B. and Ishii, T. 2006. Effect of arbuscular mycorrhizal fungi on tree growth and nutrient uptake of Sclerocarya birrea under water stress, salt stress and flooding J. Jap. Soc. Hortic. Sci. 75 26-31.

Ogg C.L. 1960. Determination of Nitrogen by the Micro-Kjeldahl Method Journal of Association of Official Agricultural Chemists 43:689–693 https://doi.org/10.1093/jaoac/43.3.689

Querejeta, J.I., Barea, JM. Allen, MF. Caravac, F. and Roldan, A. 2003. Differential response of a C13 and water use efficiency to arbuscular mycorrhizal infection in two arid land woody plant species Oecology 135:510-515.

Rao, G.Y.S., Bagyaraj, D. S. and Rai PV. 1983. Selection of efficient VA mycorrhizal fungus for finger millet. Zbl. Mikrobiology 138:409-413.

Rutto, L.K., Mizutani F., Asano Y., and Kadoya K. 2002a. Effect of inoculation with arbuscular mycorrhizal (AM) fungus on phosphorus nutrition in loquat seedlings. Bull. Exp. Farm Fac. Agr., Ehime Univ. 24: 1 – 7.

Wamocho, L.S. 1998. Studies on the use of vesicular arbuscular mycorrhizal fungi for fruit production in Kenya. PhD Thesis, Jomo Kenyatta University of Agriculture and Technology.

Rydlová, J., Püschel, D. Sudová, R. Gryndler, M. Mikanová, O and Vosátka, M. 2011. Interaction of arbuscular mycorrhizal fungi and rhizobia: Effects on flax yield in spoil-bank clay. Journal of Plant Nutrition and Soil Science 174:128-134.

Sah, S., Reed, S. Jayachandran, K. Dunn, C. and Fisher, J.B. 2006. The Effect of Repeated Short-term Flooding on Mycorrhizal Survival in Snap Bean Roots. Hortscience 41(3):598-602.

Schnepf, A., Leitner, D. Klepsch, S. Pellerin S. and Mollier, A. 2011. Modelling phosphorus dynamics in the soil-plant system. In Bünemann EK, Obserson A, Frossard E, eds, Phosphorus in Action: Biological Processes in Soil Phosphorus Cycling. pp 113–133 Heidelberg: Springer.

Smith, S.E. and Read, DJ. (eds.) 2008. Mycorrhizal Symbiosis. New York: Elsevier.

Smith, S.E. and Smith, F.A. 2011. Roles of arbuscular mycorrhizas in plant nutrition and growth: new paradigms from cellular to ecosystems scales. Annual Review of Plant Biology 63:227–250.

Sundar, S.K., Palavesam, A. and Parthipan, B. 2010. Effect of native dominant AM fungus and PGPRs on growth and biochemical characteristics of medicinally important Indigofera aspalathoides Vahl.ex. DC. International Journal Biology and Biotechnology 7:59–67.

Suri, V.K and Choudhary, A.K. 2013. Effects of vesicular arbuscular mycorrhizae and applied phosphorus through targeted yield precision model on root morphology,
productivity, and nutrient dynamics in soybean in an acid alfisol. Comm Soil Science Plant Analysis 17:2587-2604.
Tas, B. 2014. Effect of the Mycorrhiza Application on the Agronomical Properties of Sweet Corn Varieties. Journal of Agriculture and Allied Sciences 3(2):41.47.
Vaseghmanesh, T., Kordlaghari, KP. Neia, M. and Kelidari, A. 2014. The response of yield components of sunflower to mycorrhiza inoculation and phosphorus fertilizer Annals of Biological Research 4(3):101-104.

Wamocho, L.S. 1998. Studies on the use of vesicular arbuscular mycorrhizal fungi for Fruit production in Kenya. P
h.D thesis, Juja: Jomo Kenyatta University of Agriculture and Technology.

Yaseen, T., Burni, T. and Hussain, F. 2012. Effect of Arbuscular Mycorrhizal inoculation on nutrient uptake, growth and Productivity of chickpea (Cicer arietinum) varieties. International Journal of Agronomy and Plant Production 3(9):334.345.
Published
2021-05-09
How to Cite
Chebet, D., , Wanzala, F., & Kariuki, W. (2021, May 9). Vesicular Arbuscular Mycorrhizal Inoculation Influences Growth, Nutrient Absorption and Hyphae Colonization of Rough Lemon (Citrus limon) Seedlings. African Journal of Education,Science and Technology, 6(3). Retrieved from http://ajest.info/index.php/ajest/article/view/567
Section
Articles